ANNOTATED BIBLIOGRAPHY
Principles of Rotary Cell Culture System (RCCS)
Cummings LJ, Sawyer NB, Morgan SP, Rose FR, Waters SL. Tracking large solid constructs suspended in a rotating bioreactor: A combined experimental and theoretical study. Biotechnol Bioeng 104:1224-1234, 2009.
Cummings LJ, Waters SL. Tissue growth in a rotating bioreactor. Part II: fluid flow and nutrient transport problems. Math Med Biol 24: 169-208, 2007.
Rivera-Solorio I, Kleis SJ: Model of the mass transport to the surface of animal cells cultured in a rotating bioreactor operated in micro gravity. Biotechnol Bioeng. 94: 495-504, 2006.
Botchwey EA, Pollack SR, Levine EM, Johnston ED, Leurencin CT: Quantitative Analysis of Three- Dimensional Fluid Flow in Rotating Bioreactors for Tissue Engineering. J Biomed Mater Res 69: 205-215, 2004.
Lappa M: Organic Tissues in Rotating Bioreactors: Fluid-Mechanical Aspects, Dynamic Growth Models, and Morphological Evolutions. Biotechnol Bioeng. 84: 518-532, 2003.
Hammond TG, and Hammond JM: Optimized Suspension Culture: The Rotating-Wall Vessel. Am J Physiol Renal Physiol 281: F12-F25, 2001.
Reviews
Lee KW, Wang S, Dadsetan M, Yaszemski MJ, Lu L. Enhanced cell ingrowth and proliferation through three dimensional nano composite scaffolds with controlled pore structures. Biomacromolecules. 11:682-9, 2010.
Barrila J, Radtke AL,Crabbe A, Sarker SF, Herbst-Kralovetz MM, Ott, CM, Nickerson CA. Organotypic 3D cell culture models: using the rotating wall vessel to study host-pathogen interactions. Nature Rev Microbiol 8:791-801, 2010.
Navran S. The application of low shear modeled microgravity to 3-D cell biology and tissue engineering. Biotechnol Ann Rev. 14: 275-296, 2008.
Nickerson CA, Richter EG, Ott CM. Studying host-pathogen interactions in 3-D: organotypic models for infectious disease
and drug development. J Neuroimmune Pharmacol. 2007 2:26-31, 2007.
Nickerson CA, Ott CM: A New Dimension in Modeling Infectious Disease. ASM News 70: 169-175, 2004.
Nickerson CA, Ott CM, Wilson JW, Ramamurthy R, LeBlanc CL,et al.: Low-Shear Modeled Microgravity: A Global Environmental Regulatory Signal Affecting Bacterial Gene Expression, Physiology, and Pathogenesis. Journal of Microbiol Methods 54:1-11, 2003.
Biomaterials
Skardal A., Sarker SF, Crabbe A, Nickerson CA, Prestwich GD. The generation of 3-D tissue models based on hyaluronan hydrogel-coated microcarriers within a rotating wall bioreactor. Biomaterials 31:8426-8435, 2010.
Lv Q, Nair L, Laurencin CT. Fabrication, characterization, and in vitro evaluation of poly(lactic acid glycolic acid)/nano-hydroxyapatite composite microsphere-based scaffolds for bone tissue engineering in rotating bioreactors. J Biomed Mater Res A 91:679-691, 2009.
Li WJ, Jiang, YJ, Tuan RS. Cell-Nanofiber based cartilage tissue engineering using improved cell seeding, growth factor and bioreactor technologies. Tissue Engineering: Part A. 14: 639-48, 2008.
Song K, Yang Z, Liu T, Zhi W, Li X, Deng L, Cui Z, Ma X. Fabrication and detection of tissue-engineered bones with bioderived scaffolds in a rotating bioreactor. Biotechnol. Appl. Biochem. 45(pt 2): 65-74, 2006.
Akmal M, Anand A, Anand B, Wiseman M, Goodship AE, Bentley G: The culture of articular chondrocytes in hydrogel constructs within a bioreactor enhances cell proliferation and matrix synthesis. J Bone Joint Surg Br 88: 544-553, 2006.
Bone
Ko YJ, Zaharias RS, Seabold DA, Lafoon JE, Schneider GB. Analysis of the attachment and differentiation of three-dimensional rotary wall vessel cultured human preosteoblasts on dental implant surfaces. Int J Oral Maxilofac Implants 25:722-728, 2010.
Jin F, Zhang Y, Xuan K, He D, Deng T, Tang L, Lu W, Duan Y. Establishment of three-dimensional tissue- engineered bone constructs under microgravity-simulating conditions. Artif Organs 34: 118-125, 2009.
Saxena R, McDonald J. Osteoblast and Osteoclast Differentiation in Modeled Microgravity. Ann NY Acad Sci.
1116: 494-498, 2007.
Yoshioka T, Mishima H, Ohyabu Y, Sakai S, Akaogi H, Ishii T, Kojima H, Tanaka J, Ochiai N, Uemura T. Repair of large osteochondral defects with allogenic cartilaginous aggregates formed from bone marrow-derived cells using RWV bioreactor. J Orthop Res 25:1291-1298, 2007.
Bucaro MA, Zahm AM, Risbud MV, Avyaswamy PS, Mukundakrishnan K, Steinbeck MJ, Shapiro IM, Adams CS. The effect of simulated microgravity on osteoblasts is independent of the induction of apoptosis. J Cell Biochem 102: 483-495, 2007.
Zheng Q, Huang G, Xu Y, Guo C, Xi Y, Pan Z, Wang J. Could the effect of modeled microgravity on osteogenic differentiation of human mesenchymal stem cells be reversed by regulation of signaling pathways? Biol Chem 388: 755-763, 2007.
Pound JC, Green DW, Roach HI, Mann S, Oreffo RO. An ex vivo model for chondrogenesis and osteogenesis.
Biomaterials 28:2839-2849, 2007.
Rucci N, Rufo A, Alamanou M, Teti A. Modeled microgravity stimulates osteoclastogenesis and bone resorption by increasing osteoblast RANK/OPG ratio. J. Cell. Biochem. 100: 464-473, 2007.
Marolt D, Augst A, Freed LE, Vepari C, Fajardo R, Patel N, Gray M, Farley M, Kaplan D, Vunjak-Novakovic G. Bone and cartilage tissue constructs grown using human bone marrow stromal cells, silk scaffolds and rotating bioreactors. Biomaterials 27: 6138-6149, 2006.
Klement BJ, Young QM, George BJ, Nokkaew M: Skeletal Tissue Growth, Differentiation, and Mineralization in the NASA Rotating Wall Vessel. Bone 34: 487-498, 2004.
Botchwey EA, Pollack SR, El-Amin S, Levine EM, Tuan RS and Laurencin CT: Human Osteoblast-Like Cells in
Three-Dimensional Culture with Fluid Flow. Biorheology 40:299-306, 2003.
Rucci N, Migliaccio S, Zani BM, Taranta A, and Teti A, Characterization of the Osteoblast-like Cell Phenotype Under Microgravity Conditions in the NASA-approved Rotating Wall Vessel Bioreactor (RWV), J Cell Biochem 85:167-179, 2002.
CANCER
Marrero B, Messina JL, Heller R. Generation of a tumor spheroid in a microgravity environment as a 3D model of melanoma. In Vitro Cell Dev Biol Anim. 45: 523-34, 2009.
Vertrees RA, McCarthy M, Solley T, Popov VL, Roaten J, Pauley M, Wen X, Goodwin TJ. Development of a three-dimensional model of lung cancer using cultured transformed lung cells. CancerBiol Ther 8:356-365, 2009.
Lawrenson K, Benjamin E, Turmaine M, Jacobs I, Gayther S, Dafou D. In vitro three-dimensional modelling of human ovarian surface epithelial cells. Cell Prolif. 42:385-93, 2009.
Grun B, Benjamin E, Sinclair J, Timms JF, Jacobs IJ, Gayther SA, Dafou D. Three-dimensional in vitro cell biology models of ovarian and endometrial cancer. Cell Prolif. 42:219-28, 2009.
Sung, S-Y, Hsieh, C-L, Law A, Zhau HE, Pathak S, A Multani AS, Lim S, Coleman IM, Wu L-C, Figg WD, Dahut WL, Nelson P, Lee JK, Amin MB, Lyles R, Johnstone PAJ, Marshall FF, Chung LWK. Coevolution of Prostate Cancer and Bone Stroma in Three-Dimensional Coculture: Implications for Cancer Growth and Metastasis. Cancer Res 68:9996-10003, 2008.
Becker JL, Blanchard DK. Characterization of primary breast carcinomas grown in three-dimensional cultures. J Surg Res 142: 256-262, 2007.
Vamvakidou AP, Mondrinos MJ, Petushi SP, Garcia FU, Lelkes PI, Tozeren A. Heterogeneous breast tumoroids: An in vitro assay for investigating cellular heterogeneity and drug delivery. J Biomol Screen 12:13-20, 2007.
Taga M, Yamauchi K, Odle J, Furian L, Sundaresan A, Ramesh GT, Pellis NR, Andrassy RJ, Kulkarni AD. Melanoma growth and tumorigenicity in models of microgravity. Aviat. Space Environ. Med. 77: 1113-1116, 2006.
Vincent L, Avancena P, Cheng J, Rafii S, Rabbany S,: Simulated Microgravity Impairs Leukemic Cell Survival Through Altering VEGFR-2/VEGF-A Signaling Pathway: Annals of Biomedical Engineering, 33: 1405-1410, 2005.
Song H, David O, Clejan S, Giordano CL, Pappas-Lebeau H, Xu, L, O’Connor KC: Spatial Composition of Prostate Cancer Spheroids in Mixed and Static Cultures. Tissue Eng. 10: 7/8, 1266-1276, 2004.
Green LM, Patel Z, Murray DK, Rightnar S, Burell CG, Gridley DS, Nelson GA: Cytoskeletal and Functional Changes in Bioreactor Assembled Thyroid Tissue Organoids Exposed to Gamma Radiation. J Radiat. Res. 43 (supplement): S213-S218, 2002.
Winkenwerder JJ, Palechek PL, Reece JS, Saarinen MA, Arnold MA, Cohen MB, Murhammer DW: Evaluating Prostate Cancer Cell Culturing Methods: A Comparison of Cell Morphologies and Metabolic Activity. Oncol.. Rep. 10: 783-789, 2003.
Nakamura K, Kuga H, Morisaki T, Baba E, Sato N, Mizumoto K, Sueshi K, Tanaka M, and Katano M: Simulated Microgravity Culture System for a 3-D Carcinoma Tissue Model Biotechniques 33:1068-1076, 2002.
Rhee HW, Shau HE, Pathak S, Multani AS, Oennanen S, Visakorpi T, and Chung LWK: Permanent Phenotypic and Genotypic Changes of Prostate Cancer Cells Cultured In a Three-Dimensional Rotating-Wall Vessel. In Vitro Cell Dev Biol Anim 37: 127-140, 2001.
Licato LL, Prieto VG, and Grimm EA: A Novel Preclinical Model of Human Malignant Melanoma Utilizing Bioreactor Rotating-Wall Vessels. In Vitro Cell Dev Biol Anim 37: 121-126, 2001.
Jessup JM, Frantz M, Sonmez-Alpan E, Locker J, Skena K, Waller H, Battle P, Nachman A, Bhatti, Weber ME, Thomas DA, Curbeam RL, Baker TL, and Goodwin TJ: Microgravity Culture Reduces Apoptosis and Increases the Differentiation of a Human Colorectal Carcinoma Cell Line, In Vitro Cell Dev Biol 36:367-373, 2000.
Chopra V, Dinh TV, and Hannigan EV: Three-Dimensional Endothelial-Tumor Epithelial Cell Interactions in Human Cervical Cancers. In Vitro Cell Dev Biol Anim 33: 432-42, 1997.
Jessup JM, Brown D, Fitzgerald W, Ford RD, Nachman A, Goodwin TJ and Spaulding G: Induction of Carcinoembryonic Antigen Expression in a Three-Dimensional Culture System. In Vitro Cell Dev Biol Anim 33: 352-7, 1997.
Becker JL, Papenhausen PR and Widen RH: Cytogenetic, Morphologic and Oncogene Analysis of a Cell Line Derived from a Heterologous Mixed Mullerian Tumor of the Ovary. In Vitro Cell Dev Biol Anim 33: 325-31, 1997.
Chondrocytes
Villanueva I, Klement BJ, Von Deutsch D, Bryant SJ. Cross-linking density alters early metabolic activities in chondrocytes encapsulated in poly(ethylene glycol) hydrogels and cultured in the rotating wall vessel. Biotechnol Bioeng. 102:1242-50, 2009.
Sakai S, Mishima H, Ishii T, Akaogi H, Yoshioka T, Ohyabu Y, Chang F, Ochiai N, Uemura T. Rotating three- dimensional dynamic culture of adult human bone marrow-derived cells for tissue engineering of hyaline cartilage. J Orthop Res.27:517-21, 2009.
Li WJ, Jiang YJ, Tuan RS. Cell-Nanofiber-Based Cartilage tissue Engineering Using Improved Cell Seeding, Growth Factor, and Bioreactor Technologies. Tissue Eng Part A 14: 639-648, 2008.
Pei M, He F, Kish VL, Vunjak-Novakovic G. Engineering of Functional Cartilage Tissue Using Stem Cells from Synovial Lining: A Preliminary Study. Clin Orthop Relat Res 466: 1880-1889, 2008.
Pound JC, Green DW, Roach HI, Mann S, Oreffo RO. An ex vivo model for chondrogenesis and osteogenesis.
Biomaterials 28:2839-2849, 2007.
Ohyabu Y, Kida N, Kojima H, Taguchi T, Tanaka J, Uemura T. Cartilaginous tissue formation from bone marrow using rotating wall vessel (RWV) bioreactor. Biotechnol.Bioeng.95:1003-1008, 2006.
Marolt D, Augst A, Freed LE, Vepari C, Fajardo R, Patel N, Gray M, Farley M, Kaplan D, Vunjak-Novakovic G. Bone and cartilage tissue constructs grown using human bone marrow stromal cells, silk scaffolds and rotating bioreactors. Biomaterials 27: 6138-6149, 2006.
Hu JC, Athanasiou KA: Low-density Cultures of Bovine Chondrocytes: Effects of Scaffold Material and
Culture System. Biomaterials 26:2001-2012, 2005.
Marlovits S, Tichy B, Truppe M, Gruber D, Vecsei V. Chondrogenesis of Aged Human Articular Cartilage in a
Scaffold-Free Bioreactor. Tissue Eng. 9: 1215-1226, 2003.
Marlovits S, Tichy B, Truppe M, Gruber D, Schlegel W. Collagen Expression in Tissue Engineered Cartilage of
Aged Human Articular Chondrocytes in a Rotating Bioreactor: Int. Jour. Artificial Organs 26: 319-330, 2003.
Darling EM, Athanasiou KA: Articular Cartilage Bioreactors and Bioprocesses. Tissue Eng. 9: 9-26, 2003.
Pei M, Solchaga LA, Seidel J, Zenf L. Vunjak-Novakovic G, Caplan AI, Freed LE. Bioreactors Mediate the
Effectiveness of Tissue Engineering Scaffolds: FASEB J 16:1691-4, 2002.
Pei M, .Seidel J, G.Vunjak-Novakovic,and.Freed L.E. Growth factors for sequential cellular de-and re-di fferentiation in tissue engineering: Biochem Biophysl Res Commun 294, 149-154, 2002.
Vunjak-Novakovic G, Obradovic B, Martin I, Freed LE: Bioreactor Studies of Native and Tissue Engineered
Cartilage. Biorheology 39:259-268, 2002.
Koch RJ, Gorti GK: Tissue Engineering with Chondrocytes, Facial Plast Surg 18:59-68, 2002.
CARDIOVASCULAR
Kwon O, Tranter M, Jones WK, Sankiovic JM, Banerjee RK. Differential translocation of nuclear factor-kappaB
in a cardiac muscle cell line under gravitational changes. J Biomech Eng. 131:064503, 2009.
Lu S, Liu S, He W, Duan C, Li Y, Liu Z, Hao T, Wang Y, Li D, Wang C, Gao S. Bioreactor Cultivation
Enhances NTEB Formation and Differentiation of NTES Cells into Cardiomyocytes. Cloning Stem Cells
10:363-370, 2008.
Arrigoni C, Chitto A, Mantero S, Remuzzi A. Rotating versus perfusion bioreactor for the culture of engineered vascular constructs based on hyaluronic acid. Biotechnol Bioeng. 100: 988-997, 2008.
Bruno S, Bussolati B, Scacciatella P, Marra S, Sanavio F, Tarella C, Camussi G. Combined administration of G- CSF and GM-CSF stimulates monocyte-derived pro-angiogenic cells in patients with acute myocardial infarction. Cytokine 34: 56-65, 2006.
Guo XM, Zhao YS, Chang HX, Wang CY, Ling-Ling E, Zhang XA, Duan CM, Dong LZ, Jiang H, Li J, Song Y, Yang XJ. Creation of engineered cardiac tissue in vitro from mouse embryonic stem cells. Circulation 113 :2229-2237, 2006.
Bursac N, Papadaki M, White JA, Eisenberg SR, Vunjak-Novakovic G, Freed L: Cultivation in Rotating Bioreactors Promotes Maintenance of Cardiac Myocyte Electrophysiology and Molecular properties. Tissue Eng. Vol. 9, No.6, 1243-1253, 2003.
Van Luyn MA, Tio RA, Gallego y van Seijen XJ, Plantinga JA, de Leij LFMH, DeJongste ML, van Wachem PB: Cardiac Tissue Engineering: Characteristics of in Unison Contracting Two- and Three-dimensional Neonatal Rat Ventricle Cell (Co)-Cultures. Biomaterials 23: 4793-4801, 2002.
Sutherland FW, Perry TE, Nassen BA, Wang J, Kaushal S, Guleserian KJ, Martin DP, Vacanti JP and Mayer JE: Advances in the Mechanisms of Cell Delivery to Cardiovascular Scaffolds: Comparison of Two Rotating Cell Culture Systems. ASAIO J 48:346-9, 2002.
Papadaki M, Bursac N, Langer R, Merok J, Vunjak-Novakovic G, Freed LE: Tissue Engineering of Functional
Cardiac-Muscle: Molecular, Structural, and Electrophysiological Studies. Am J Physiol Heart Physiol
280:H168-178, 2001.
Lwigale PY, Thurmond JE, Norton WN, Spooner BS, Wiens DJ: Simulated Microgravity and Hypergravity
Attenuate Heart Tissue Development in Explant Culture. Cells Tissues Organs 167: 171-183, 2000.
Carrier RL, Papadaki M, Rupnick M, Schoen F, Bursac N, Langer R, Freed LE, Vunjak-Novakovic G: Cardiac Tissue Engineering: Cell Seeding, Cultivation Parameters, and Tissue Construct Characterization. Biotechnol Bioeng 64: 580-589, 1999.
DENTAL
Li S, Ma Z, Niu Z, Qian H, Xuan D, Hou R, Ni L. NASA approved rotary bioreactor enhances proliferation and osteogenesis of human periodontal ligament stem cells. Stem Cells Dev. 18:1273-82, 2009.
Inac B, Eser Elcin A, Koc A, Balos K, Parlar A, Murat Elcin Y. Encapsulation and osteoinduction of human periodontal ligament fibroblasts in chitosan-hydroxyapatite microspheres. Biomed Mater Res A 82: 917-926, 2007.
Inanc B, Elcin AE, Elcin YM: Osteogenic Induction of Human Periodontal Ligament Fibroblasts Under Two- and Three- Dimensional Culture Conditions. Tissue Eng. 12: 257-266, 2006.
ENDOTHELIAL CELL CULTURE
Cotrupi S, Ranzani D, Maier JA: Impact of modeled microgravity on microvascular endothelial cells. Biochem
Biophys Acta 1746(2):163-168, 2005.
Carrlsson SI, Bertilaccio MT, Ascari I, Bradamante S, Maier JA: Modulation of Human Endothelial Cell
Behavior in Simulated Microgravity. J Gravit Physiol 9: P273-274, 2002.
Sanford GL, Ellerson D., Melhado-Gardner C., Sroufe AE., Harris-Hooker S: Three-dimensional growth of endothelial cells in the microgravity-based rotating wall vessel bioreactor. In Vitro Cell Dev Biol Anim. 38(9):493-504, 2002.
HEMATOPOIETIC SYSTEM
Singh KP, Kumari R, Dumond JW. Simulated microgravity-induced epigenetic changes in human lymphocytes.
J Cell Biochem 111:123-129, 2010.
Simons DM, Gardner EM, Lelkes PI. Intact T cell receptor signaling by CD4(+) T cells cultured in the rotating wall-vessel bioreactor. J Cell Biochem. 109:1201-9, 2010.
Singh KP, Kumari R, Dumond JW. Simulated microgravity-induced epigenetic changes in human lymphocytes.
J Cell Biochem 111:123-129, 2010.
Sundaresan A, Pellis NR. Cellular and genetic adaptation in low-gravity environments. Ann N Y Acad Sci.
1161:135-46, 2009.
Li X, Liu CT, Zhou H. The influence of leptin on the activity of lung lymphocytes under simulated microgravity. Eur J App Physiol. 107: 335-44, 2009.
Simmons DM, Gardner EM, Lelkes PI. Sub-mitogenic phorbol myristate acetate co-stimulation rescues the PHA-induced activation of both naïve and memory T cells cultured in the rotating-wall vessel bioreactor. Cell Biol Intl. 33:882-6, 2009.
Kumari R, Singh KP, Dumond JW Jr. Simulated microgravity decreases DNA repair capacity and induces DNA
damage in human lymphocytes. J. Cell Biochem.107:723-31, 2009.
Ward NE, Pellis NR, Risin SA, Risin D. Gene expression alterations in activated human T-cells induced by modeled microgravity. J. Cell. Biochem. 99: 1187-1202, 2006.
Simons DM, Gardner EM, Lelkes PI. Dynamic culture in a rotating-wall vessel bioreactor differentially inhibits murine T-lymphocyte activation by mitogenic stimuli upon return to static conditions in a time- dependent manner. J Appl Physiol 100: 1287-1292, 2006.
Ritz BW, Lelkes PI, Gardner EM. Functional recovery of peripheral blood mononuclear cells in modeled microgravity. FASEB J. 20: 305-307, 2006.
Plett PA, Abonour R, Frankovitz SM, Orschell CM. Impact of Modeled Microgravity on Migration, Differentiation, and Cell Cycle Control of Primitive Human Hematopoietic Progenitor Cells. Experimental Hematology 32: 773-781, 2004.
Bakos A, Varkonyi A, Minarovits J, Batkai . Effect of Simulated Microgravity on the Production of IL-12 by PBMC’s. J Gravit Physiol. 9: 293-294, 2002.
Licato LL, Grimm EA. Multiple Interleukin-2 Signaling Pathways Differentially Regulated by Microgravity.
Immunopharmacology 44: 273-9, 1999.
INSECT
Joosten CE, Shuler ML. Effect of Culture Conditions on the Degree of Sialylation of a Recombinant
Glycoprotein Expressed in Insect Cells. Biotechnol. Prog. 19: 739-749, 2003.
Saarinen MA and Murhammer DW. Culture in the Rotating-Wall Vessel Affects Recombinant Protein
Production Capability of Two Insect Cell Lines in Different Manners. In Vitro Cell Dev Biol Anim 36: 362-366,
2000.
Cowger NL, O’Connor KC, Bivins J. Influence of Simulated Microgravity on the Longevity of Insect-Cell
Culture. Enzyme and Microbial Technology 20: 326-332, 1997.
HEPATIC/PANCREAS
Nelson LJ, Walker SW, Hayes PC, Plevris JN. Low-shear modeled microgravity environment maintains morphology and differentiated functionality of primary porcine hepatocyte cultures. Cells Tissues Organs
192:125-140, 2010.
Han X, Qui L, Zhang Y, Kong Q, Wang H, Wang H, Li H, Duan C, Wang Y, Song Y, Wang C. Transplantation of sertoli-islet cell aggregates formed by microgravity: prolonged survival in diabetic rats. Exp Biol Med (Maywood). 234:595-603, 2009.
Okamura A, Zheng YW, Hirochika R, Tanaka J, Taniguchi H. In-vitro reconstitution of hepatic tissue architecture with neonatat mouse liver cells using three –dimensional culture. J Nanosci Nanotechnol 7: 721-725, 2007.
Clement JQ, Lacy SM, Wilson BL. Genome-wide gene expression profiling of microgravity effect on human liver cells. J Gravit Physiol 14: P121-122, 2007.
Coward SM, Selden C, Mantalaris A, Hodgson HJ: Proliferation Rates of HepG2 Cells Encapsulated in Alginate
Are Increased in a Microgravity Environment Compared With Static Cultures. Artif Organs 29: 152-158,
2005.
Song C, Duan XQ, Li X, Han LO, Xu P, Song CF, Jin LH. Experimental Study on Islet Cells in Rats Under
Condition of Three-dimensional Microgravity. Zhonghua Wai Ke Za Zhi 42: 559-561, 2004.
Brown LA, Arterburn LM, Miller AP, Cowger NL, Hartley SM, Andrews A, Silber PM, Li AP. Maintenance of Liver Functions in Rat Hepatocytes Cultured as Spheroids in a Rotating Wall Vessel. In Vitro Cell Dev Biol Anim Jan; 39: 13-20,2003.
Cameron DF, Hushen JJ, Dejarlais T, Colado L, Wolski KM, Sanberg PR, Saporta S. A Unique Cytoplasmic
Marker for Extratesticular Sertoli Cells, Cell Transplant 11: 507-512, 2002.
Khaoustov VI, Risin D, Pellis NR, Yoffe B. Microarray Analysis of Genes Differentially Expressed in HEPG2
Cells Cultured in Simulated Microgravity: Preliminary Report. In Vitro Cell Dev Biol Anim 37: 84-8, 2001.
Dabos KJ, Nelson LJ, Bradnock TJ, Parkinson JA, Sadler IH, Hayes PC, Plevris JN. The Simulated Microgravity Environment Maintains Key Metabolic Functions and Promotes Aggregation of Primary Porcine Hepatocytes. Biochem Biophys Acta 1526: 119-130, 2001.
Yoffe B, Darlington GJ, Soriano HE, Krishman B, Risin D, Pellis NR, Khaustov VI. Cultures of Human Liver
Cells in Simulated Microgravity Environment. Adv Space Res 24: 829-836, 1999.
Khaoustov VI, Darlington GJ, Soriano HE, Krishnan B, Risen D, Pellis NR, Yoffe B. Induction of Three- Dimensional Assembly of Human Liver Cells by Simulated Microgravity. In Vitro Cell Dev Biol Animal, 35:
501-509. 1999.
MICROBIOLOGY
Crabbe A, Pycke B, Van Houdt R, Monsieurs P, Nickerson C, Leys N, Cornelis P. Response of Pseudomonas aeruginosa PAO1 to low shear modeled microgravity involves AlgU regulation. Environ Microbiol 12:1545-
1564, 2010.
Beuls E, Van Houdt R, Leys Y, Dijkstra C Larkin O, Mahillon J. Bacillus thuringiensis conjugation in simulated microgravity. Astrobiology. 9: 797-805, 2009.
Crabbé A, De Boever P, Van Houdt R, Moors H, Mergeay M, Cornelis P. Use of the rotating wall vessel technology to study the effect of shear stress on growth behaviour of Pseudomonas aeruginosa PA01. Environ Microbiol 10: 2098-2110, 2008.
Wilson JW, Ott CM, Zu Bentrup KH, Ramamurthy R, Quick L, Porwollik S, Cheng P, McClelland M, Tsaprailis G, Radabaugh T, Hunt A, Fernandez D, Richter E, Shah WM, Kilcoyne M, Joshi L, Nelman-Gonzalez M, Hing S, Parra M, Dumars P, Norwood K, Bober R, Devich J, Ruggles A, Goulart C, Rupert M, Stodieck L, Stafford P, Catella L, Schurr MJ, Buchanan K, Morici L, McCracken J, Allen P, Baker-Colman C, Hammond T, Vogel J, Nelson R, Pierson DL, Stefanyshyn-Piper HM, Nickerson CA. Space flight alters bacterial gene expression and virulence and reveals a role for global regulator Hfq. Proc Natl Acad Sci USA 104: 16299-16304, 2007.
Tucker DL, Ott CM, Huff, S, Fofanov V, Willson RC, Fox GE. Characterization of Escherichia coli MG1655 grown in a low shear modeled microgravity environment. BMC Microbiology 7: 15, 2007.
Smith YC, Grande KK, Rasmussen SB, O’Brien AD. Novel three-dimensional organoid model for evaluation of the interaction of uropathologenic Escherichia coli with terminally differentiated human urothelial cells. Infect. Immun. 74: 750-757, 2006.
Lynch SV, Mukundakrishnan K, Benoit MR, Ayyaswamy PS, Matin A. Escherichia coli biofilms formed under low-shear modeled microgravity in a ground-based system. Appl. Environ. Microbiol. 72: 7701-7710, 2006.
Honer Zu Bentrup K, Ramamurthy R, Ott CM, Emami K, Nelman-Gonzalez M, Wilson JW, Richter EG, Goodwin TJ, Alexander JS, Pierson DL, Pellis N, Buchanan KL, Nickerson CA. Three-dimensional organotypic models of human colonic epithelium to study the early stages of enteric salmonellosis. Microbes Infect. 8: 1813-182,
2006.
Carvalho HM, Teel LD, Goping G, O’Brien AD. A three-dimensional tissue culture model for the study of attach and efface lesion formation by enteropathic and enterohaemorrhagic Escherichia coli. Cell Microbiol
7: 1771-1781, 2005.
Carterson AJ, Honer zu Bentrup K, Ott CM, Clarke MS, Pierson DL, Vanderburg CR, Buchanan KL, Nickerson CA, Schurr MJ. A549 Lung Epithelial Cells Grown as Three-Dimensional Aggregates: Alternative Tissue Culture Model for Pseudomonas Aeruginosa Pathogenesis. Infect Immun 73: 1129-1140, 2005.
Duray P, Yin S, Ito Y, Bezrukov L, Cox C, Cho M, Fitzgerald W. Invasion of Human Tissue Ex Vivo by
Borrelia: Journal of infectious Diseases 191:1747-1754, 2005.
LaMarca H.L, Ott C.M, Höner zu Bentrup K, LeBlanc C.L, Pierson D.L, Nelson A.B, Scandurro A.B, Whitley G.St.J, Nickerson C.A, and C.A.Morris. Three-Dimensional Growth of Extravillous Cytotrophoblasts Promotes Differentiation and Invasion. Placenta 26::709-720, 2005.
Lynch SV, Brodie EL, Matin A. Role and Regulation of Sigma S in General Resistance Conferred by Low- shear Simulated Microgravity in Escherichia Coli. J Bacteriol 186: 8207-8212, 2004.
Nickerson CA, Ott CM, Wilson JW, Ramamurthy R, Pierson DL. Microbial Responses to Microgravity and
Other Low-Shear Environments. Microbiol Mol Biol Rev Jun; 68: 345-61, 2004.
Gorzelak M, Trevors JT. Growth and Membrane Polarization in Pseudomonas aeruginosa UG2
Grown in Randomized Microgravity in a High Aspect Ratio Vessel. Biochimica et Biophysica Acta 1624: 76-
81, 2003.
Nickerson CA, Ott CM, Wilson JW, Ramamurthy R, LeBlanc CL,et al. Low-Shear Modeled Microgravity: A Global Environmental Regulatory Signal Affecting Bacterial Gene Expression, Physiology, and Pathogenesis. Journal of Microbiological Methods 54:1-11, 2003.
Wilson JW, Ott CM, Ramamurthy R, Porwollik S, McClelland M, Pierson DL, Nickerson CA. Low-Shear Modeled Microgravity Alters the Salmonella Enterica Serovar Typhimurium Stress Response in an RpoS- independent Manner. Applied and Environmental Microbiology 68:5408-5416, 2002.
Wilson JW, Ramamurthy R, Porwollik S, McClelland M, Hammond T, Allen P, Ott CM, Pierson DL, Nickerson CA. Microarray Analysis Identifies Salmonella Genes Belonging to the Low-Shear Modeled Microgravity Regulon, Proc. Natl. Acad. Sci. USA 99: 13807-13812, 2002.
Nickerson CA, Goodwin TJ, Terlonge J, Ott CM, Buchanan KL, Uicker WC, Emami K, LeBlanc C, Ramamurthy R, Clarke MS, Vanderburg CR, Hammond T, Pierson DL. Three-Dimensional Tissue Assemblies: Novel Models for the Study of Salmonella Enterica Serovar Typhimurium Pathogenesis. Infect Immun 69: 7106-7120, 2001.
Nickerson CA, Ott M, Mister SJ, Morrow BJ, Burns-Keliher L, Pierson DL. Microgravity as a Novel
Environmental Signal Affecting Microbial Virulence. Infect. Immun. 68: 3147-3150, 2000.
Fang A, Pierson DL, Mishra SK, Demain AL. Relief from Glucose Interference in Microcin B17 Biosynthesis by
Growth in a Rotating-Wall Bioreactor. Lett Appl Microbiol 31: 39-41, 2000.
NEURAL
Ma W, Tavakoli T, Chen S, Maric D, Liu JL, O'Shaughnessy TJ. Reconstruction of Functional Cortical-Like
Tissues from Neural Stem and Progenitor Cells. Tissue Eng Part A 14: 1687-1697, 2008.
Hahn H, Muller M, Lowenheim H. Whole organ culture of the postnatal sensory inner ear in simulated microgravity. J Neurosci Methods 171: 60-71, 2008.
Shamekh R, Cameron DF, Willing AE, Saporta S. The role of connexins in the differentiation of NT2 cells in
Sertoli-NT2 cell tissue constructs grown in the rotating wall bioreactor. Exp Brain Res 170: 277-284, 2006.
Saporta S, Willing AE, Shamekh R, Bickford P, Paredes D, Cameron DF. Rapid Differentiation of NT2 Cells in a
Sertoli-NT2 Cell Tissue Constructs Grown in the Rotating Wall Bioreactor. Brain Res Bull 64: 347-356, Dec
2004.
Lin HJ, O’Shaughnessy TJ, Kelly J, Ma W. Neural Stem Cell Differentiation in a Cell-collagen-bioreactor
Culture System. Develop. Brain Res 153: 163-173, 2004.
PANCREATIC
Stepkowski SM, Phan T, Zhang H, Bilinski S, Kloc M, Qi Y, Katz SM, Rutzky LP. Immature syngeneic dendritic cells potentiate tolerance to pancreatic islet allografts depleted of donor dendritic cells in microgravity culture condition. Transplantation 82:1756-1763, 2006.
Murray H.E., Padget, M.B, Downing, R. Preservation of glucose responsiveness in human islets maintained in a rotational cell culture system. Molecular and Cellular Endocrinology 238: 39-49, 2005.
Song C, Duan XQ, Li X, Han LO, Xu P, Song CF, Jin LH. Experimental Study of Rat Beta Islet Cells Cultured under Simulated Microgravity Conditions. Acta Biochim Biophys (Shanghai) 36: 47-50, 2004.
Rutzky L, Bilinzki Z, Kloc M, Phan T, Zhang H, Katz S, Stepkowski S: Microgravity Culture Conditions
Reduces Immunogenicity And Improves Function Of Pancreatic Islets. Transplantation 74: 13-21, 2002.
PARASITOLOGY
Warren CA, Destura, RV, Sevilleja JEAD, Barroso LF, Carvalho H, Barrett LJ, O’Brien, AD, Guerrant RL.
Detection of Epithelial-Cell Injury, and Quantification of Infection, in the HCT-8 Organoid Model of Cryptosporidiosis. J Infect Dis 198: 143-149, 2008.
Garzoni LR, Adesse D, Soares MJ et al. Fibrosis and Hypertrophy Induced by T. cruzi in a Three-dimensional Cardiomyocyte-Culture System. J Infect Dis 197: 906-15, 2008.
PROSTATE
Clejan S, O’Connor K and Rosenweig N. Tri-dimensional Prostate Cell Cultures in Simulated Microgravity and Induced Changes in Lipid Second Messengers and Signal Transduction. J Cell Mol Med 5:60-73, 2001.
Margolis L, Hatfill S, Chuaqui R, Vocke C, Emmert-Buck M, Linehan WM and Duray PH. Long Term Organ
Culture of Human Prostate Tissue in a NASA-Designed Rotating Wall Bioreactor. J Urol 161: 290-297, 1999.
PULMONARY
Zhou J, Hu L, Cui Z, Jiang X, Wang G, Krissansen GW, Sun X. Interaction of SDF-1alpha and CXCR4 plays an important role in pulmonary cellular infiltration in differentiation syndrome. Int J Hematol 91:293-302, 2010.
RENAL
Cowger NL, Benes E, Allen PL, and Hammond TG. Expression of Renal Cell Protein Markers is Dependent on
Initial Mechanical Culture Conditions, J Appl Physiol 92: 691-700, 2002.
Kaysen JH, Campbell WC, Majewski RR, Goda FO, Navar GL, Lewis FC, Goodwin TJ, Hammond TG. Select De Novo Gene and Protein Expression During Renal Epithelial Cell Culture in Rotating Wall Vessels is Shear Stress Dependent. J Memb Biol 168: 77-89, 1999.
REPRODUCTIVE
Ricci G, Esposito R, Catizone A, Galdieri M. Direct effects of microgravity on testicular function: analysis of hystological, molecular and physiologic parameters. J Endocrinol Invest 31:229-237, 2008.
SALIVARY GLAND
Lewis ML, Moriarity DM and Campbell PS. Use of Microgravity Bioreactors for Development of an In Vitro
Rat Salivary Gland Cell Culture Model. J Cell Biochem 51: 265-273, 1993.
SIGNALLING
Zhou J, Hu L, Cui Z, Jiang X, Wang G, Krissansen GW, Sun X. Interaction of SDF-1alpha and CXCR4 plays an important role in pulmonary cellular infiltration in differentiation syndrome. Int J Hematol 91:293-302, 2010.
Kumar R, Harris-Hooker S, Sanford GL. The expression of growth factors and their receptors in retinal and endothelial cells cocultured in the rotating bioreactor. Ethn Dis 18(2 Suppl): S2-44-50, 2008.
Vincent L, Avancena P, Cheng J, Rafii S, Rabbany S. Simulated Microgravity Impairs Leukemic Cell Survival
Through Altering VEGFR-2/VEGF-A Signaling Pathway: Annals of Biomedical Engineering, 33: 1405-1410,
2005.
Nickerson CA, Ott CM, Wilson JW, Ramamurthy R, LeBlanc CL,et al. Low-Shear Modeled Microgravity: A Global Environmental Regulatory Signal Affecting Bacterial Gene Expression, Physiology, and Pathogenesis. Journal of Microbiological Methods 54 1-11, 2003.
SKELETAL MUSCLE
Marquette ML, Byerly D, Sognier M. A novel in vitro three-dimensional skeletal muscle model. In Vitro Cell Dev Biol Anim. 43:255-263, 2007.
Klement BJ, Young QM, George BJ, Nokkaew M. Skeletal Tissue Growth, Differentiation, and Mineralization in the NASA Rotating Wall Vessel. Bone 34: 487-498, 2004.
SKIN
Doolin EJ, Geldziler B, Strande L, Kain M, Hewitt C. Effects of Microgravity on Growing Cultured Skin
Constructs. Tissue Eng 5: 573-582, 1999.
STEM CELLS
Li S, Ma Z, Niu Z, Qian H, Xuan D, Hou R, Ni L. NASA approved rotary bioreactor enhances proliferation and osteogenesis of human periodontal ligament stem cells. Stem Cells Dev. 18:1273-82, 2009
Hwang YS, Cho J, Tay F, Heng JY, Ho R, Kazarian SG, Williams DR, Boccaccini AR, Polak JM, Mantalaris A. The use of murine embryonic stem cells, alginate encapsulation, and rotary microgravity bioreactor in bone tissue engineering. Biomaterials 30: 499-507, 2008
Ma W, Tavakoli T, Chen S, Maric D, Liu JL, O'Shaughnessy TJ. Reconstruction of Functional Cortical-Like
Tissues from Neural Stem and Progenitor Cells. Tissue Eng Part A 14: 1687-1697, 2008
Come J, Nissan X, Aubry L, Tournois J, Girard M, Perrier AL, Peschanski M, Cailleret M. Improvement of culture conditions of human embryoid bodies using a controlled perfused and dialyzed bioreactor system. Tissue Eng Part C Methods 14: 289-298, 2008
Pei M, He F, Kish VL, Vunjak-Novakovic G. Engineering of Functional Cartilage Tissue Using Stem Cells from Synovial Lining: A Preliminary Study. Clin Orthop Relat Res 466: 1880-1889, 2008
Randle WL, Cha JM, Hwang YS, Chan KL, Kazarian SG, Polak JM, Mantalaris A. Integrated 3-Dimensional
Expansion and Osteogenic Differentiation of Murine Embryonic Stem Cells. Tissue Eng 13: 2957-2970, 2007
Chen SS, Fitzgerald W, Zimmerberg J, Kleinman HK, Margolis L. Cell-cell and cell-extracellular matrix interactions regulate embryonic stem cell differentiation. Stem Cells 25: 553-561, 2007
Philp D, Chen SS, Fitzgerald W, Orenstein J, Margolis L, Kleinman HK: Complex Extracellular Matrices
Promote Tissue-specific Stem Cell Differentiation. Stem Cells 23: 288-296, 2005.
CELL & TISSUE ENGINEERING
Zhang Z, Zhou HC, Li ZG, Pan MX, Wang Z, Gao Y. Microgravity culture of hepatocytes on cellulose/gelatin macroporous microcarrier. Nan Fang Yi Ke Da Xue Xue Bao. 30:704-7,2010
Hjelm BE, Berta AN, Nickerson CA, Arntzen CJ, Herbst-Kralovetz MM. Development and characterization of a three-dimensional organotypic human vaginal epithelial cell model. Biol Reprod. 82:617-27, 2010
Skardal A., Sarker SF, Crabbe A, Nickerson CA, Prestwich GD. The generation of 3-D tissue models based on hyaluronan hydrogel-coated microcarriers within a rotating wall bioreactor. Biomaterials 31:8426-8435, 2010
Li S, Ma Z, Niu Z, Qian H, Xuan D, Hou R, Ni L. NASA approved rotary bioreactor enhances proliferation and osteogenesis of human periodontal ligament stem cells. Stem Cells Dev. 18:1273-82, 2009
Hwang YS, Cho J, Tay F, Heng JY, Ho R, Kazarian SG, Williams DR, Boccaccini AR, Polak JM, Mantalaris A. The use of murine embryonic stem cells, alginate encapsulation, and rotary microgravity bioreactor in bone tissue engineering. Biomaterials 30: 499-507, 2008
Ma W, Tavakoli T, Chen S, Maric D, Liu JL, O'Shaughnessy TJ. Reconstruction of Functional Cortical-Like
Tissues from Neural Stem and Progenitor Cells. Tissue Eng Part A 14: 1687-1697, 2008
Li WJ, Jiang YJ, Tuan RS. Cell-Nanofiber-Based Cartilage tissue Engineering Using Improved Cell Seeding, Growth Factor, and Bioreactor Technologies. Tissue Eng Part A 14:639-648, 2008
Waters SL, Cummins LJ, Shakesheff KM, Rose FR. Tissue growth in a rotating bioreactor. Part I: mechanical stability. Math. Med. Biol. 23: 311-337, 2006
Kumar R, Dutt K: Enhanced neurotrophin synthesis and molecular differentiation in non-transformed human retinal progenitor cells cultured in a rotating bioreactor. Tissue Eng. 12: 141-158, 2006.
Su GN, Hidaka M, Kimura Y, Yamamoto G: In Situ Collagen Gelation: A New Method for Constructing Large
Tissue in Rotary Culture Vessels: In Vitro Cell Dev Biol Animl 39: 368-374, 2003.
Green LM, Patel Z, Murray DK, Rightnar S, Burell CG, Gridley DS, Nelson GA: Cytoskeletal and Functional
Changes in Bioreactor Assembled Thyroid Tissue Organoids Exposed to Gamma Radiation. J Radiat. Res.
43: S213-S218, 2002.
Sanford GL, Ellerson D, Melhado-Gardner C, Sroufe AE, Harris-Hooker S: Three-dimensional Growth of
Endothelial Cells in the Microgravity-Based Rotating Wall Vessel Bioreactor: In Vitro Cell Dev Biol Anim.,
38: 493-504, 2002.
Freed LE, Vunjak-Novakovic G: Spaceflight Bioreactor Studies of Cells and Tissues. Adv Space Biol Med
8:177-195, 2002.
Sikavitsas VI, Bancroft GN, Mikos AG: Formation of three-dimensional cell/polymer constructs for bone tissue engineering in a spinner flask and a rotating wall vessel bioreactor. J Biomed Mater Res 62: 136-148, 2002.
Gosiewska A, Rezania A, Dhanaraj S, Vyakarnam M, Zhou J, Brown L, Kong W, Zimmerman M and Geesin JC: Development of a Three-Dimensional Transmigration Assay for Testing Cell-Polymer Interactions for Tissue Engineering Applications. Tissue Eng 7: 267-77, 2001.
Martin A, Zhou A, Gordon RE, Henderson SC, Schwartz AE, Friedman EW and Davies TF: Thyroid Organoid
Formation in Simulated Microgravity: Influence of Keratinocyte Growth Factor. Thyroid 10: 481-487, 2000.
Freed LE and Vunjak-Novakovic G: Tissue Engineering Bioreactors. Principles of Tissue Engineering, 2nd
Edition, Chapter 13, pp. 143-156, 2000.
Langer RS and Vacanti JP: Tissue Engineering: The Challenges Ahead. Scientific American. 280: 86-89, 1999.
Freed LE and Vunjak-Novakovic G: Culture of Organized Cell Communities. Adv Drug Delivery Reviews 33: 15-30, 1998.
Unsworth BR, Lelkes PI. The Use of Rotating Wall Bioreactors for the Assembly of Differentiated Tissue-Like
Organoids. Advances in Tissue Engineering: New developments in cartilage, skin and bone engineering, Chapt.
2.3, pp. 113-32, 1998.
Freed LE and Vunjak-Novakovic G: Microgravity Tissue Engineering. In Vitro Cell Dev Biol Anim 33: 381-385,
1997.
VETERINARY
Nelson LJ, Walker SW, Hayes PC, Plevris JN. Low-shear modeled microgravity environment maintains morphology and differentiated functionality of primary porcine hepatocyte cultures. Cells Tissues Organs
192:125-140, 2010.
VIRAL
Sainz B Jr, TenCate V, Uprichard SL. Three-dimensional Huh7 cell culture system for the study of Hepatitis C
virus infection. Virol J 6:103, 2009.
Straub TM, Honer zu Bentrup K, Orosz-Coghlan, P, Dohnalkova A, Mayer BK, Bartholomew RA, Valdez CO, Bruckner-Lea C, Gerba CP, Abbaszadegan M, Nickerson CA, In vitro Cell Culture Infectivity Assay for Human Noroviruses. Emerging Infectious Diseases 13: 396-403, 2007.
Hughes JH and Long JP: Epstein-Barr Virus Latently Infected Cells are Selectively Deleted in Simulated- Microgravity Cultures. In Vitro Cell Dev Biol Anim 37: 223-230, 2001.
Long JP, Pierson S, and Hughes JH: Suppression of Epstein-Barr Virus Reactivation in Lymphoblastoid Cells
Cultured in Simulated Microgravity. In Vitro Cell Dev Biol Anim 35: 49-54, 1999.
MISC.
Schrader S, Kremling C, Clinger M, Lagua H, Geerling G. Cultivation of Lacrimal gland acinar cells in a microgravity environment. Br J Ophthalmol. 93: 1121-1125, 2009
Kwon O, Devarakonda SB, Sankovic JM, Banerjee RK. Oxygen transport and consumption by suspended cells in microgravity: A multiphase analysis. Biotechnol Bioeng 99:99-107, 2008
Sawyer N, Worrall L, Crowe J, Waters, S, Shakesheff K, Rose F, Morgan S. In situ monitoring of 3D in vitro cell aggregation using an optical imaging system. Biotechnol Bioeng 100:159-167, 2007
Chen J, Chen R, Gao S. Morphological characteristics and proliferation of keratocytes cultured under simulated microgravity. Artif Organs 31: 722-731, 2007
Xu Y, Sun J, Mathew G, Jeevarajan AS, Anderson MM: Continuous Glucose Monitoring and Control in a
Rotating Wall Perfused Bioreactor. Biotechnol Bioeng. 86: 473-477, 2004.
Foster LJ, Catzel D, Atwa S, Zarka M, Mahler SM: Increase in Synthesis of Human Monoclonal Antibodies by
Transfected Sp2/0 Myeloma Mouse Cell Line Under Conditions of Microgravity. Biotechnol Lett. Aug. 25:
1271-1274, 2003.
Hales NW, Yamauchi K, Martinez AA, Sundaresan A, Pellis NR and Kulkarni AD: A Countermeasure to Ameliorate Immune Dysfunction in In Vitro Simulated Microgravity Environment: Role of Cellular Nucleotide Nutrition. In Vitro Cell Dev Biol (Animal) 38(4):213-217, 2002.
Kulkarni AD, Yamauchi K. and Pellis NR. Nutrition Countermeasure and Immune Function in Microgravity. Proceedings of the 2nd Pan Pacific Basin Workshop on Microgravity Sciences, Pasadena, CA. April 2001BT- 1099:1-10, 2001.
Jessup JM, and Pellis NR: NASA Biotechnology: Cell Science in Microgravity. In Vitro Cell Dev Biol Anim 37: 2, 2001.
Savary C, Grazziuti ML, Przepiorka D, Tomasovic SP, McIntyre BW, Woodside DG, Pellis NR, Pierson DL, Rex JH: Characteristics of Human Dendritic Cells Generated in a Microgravity Analog Culture System In Vitro Cell Dev Biol Anim 37:216-222, 2001.